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The oxidative degradation study of ticagrelor
Tonnerová, Barbora ; Kubíčková, Anna (advisor) ; Křížek, Tomáš (referee)
Ticagrelor is a platelet aggregation inhibitor drug, used for treatment of patients with acute coronary syndrome. The only active metabolite, a reverse blocator of P2Y12 adenosindiphosphate receptor, is produced by hepatic metabolism. Ticagrelor is soluble in 50 % (vol.) methanol solution. The most efficient separation of ticagrelor using ultrahigh performance liquid chromatography turned out to be in gradient: (0-4 min: 20→80 % A; 4-6 min: 80 % A; 6-6,5 min: 80→20 % A; 6,5-8,5 min: 20 % A; A stands for acetonitrile). Mobile phase is composed of acetonitrile and ammonium acetate buffer (20 mmol·dm-3 and pH 8,0). Reverse stationary phase is Kinetex C18 100A column (100 x 2,1 mm, 1,7 µm; Phenomenex, USA). Retention time of ticagrelor is 4,91 min. The separation method was used to observe the oxidative degradation of ticagrelor. After 7 hours of exposition to hydrogen peroxide solution at 50 řC, 20 % of ticagrelor has degraded. One degradation product was formed at same speed as the degradation of ticagrelor had. Retention time of this degradation product is 3,95 min.
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